(O32) Transcriptome Profiling Between Normal and Lymphoma in Comparison of T-cell and B-cell Types in Dogs

Background

Several studies of B-cell lymphoma (BCL) transcriptome analyses have been reported in dogs. However, no studies have been reported about the difference between T-cell lymphoma (TCL) and BCL in dogs.

Hypothesis/Objectives

This study aims to identify differences transcriptome profiling between normal and lymphoma as well as TCL and BCL in dogs.

Animals

Ten client-owned dogs diagnosed with lymphoma in a hospital were enrolled in the patient group. Six dogs were classified as TCL, and four were BCL. Seven healthy beagles were also enrolled as the controls.

Methods

Transcriptome profiling was performed with tumor tissues obtained by ultrasound-guided fine needle aspiration or biopsy. Data analysis was performed employing the fold change, independent T-test, hierarchical clustering, gene-enrichment and functional annotation analysis, and Kyoto Encyclopedia of Genes and Genomes pathways. Statistical significance was set at P-value < 0.05.

Results

Based on the threshold criteria, several differentially expressed genes (DEGs) were identified
between normal and BCL (n=2215). There were fewer significant DEGs between the normal
and TCL (n=125), and various expression patterns were identified among each patient. Also,
DEGs such as JUN, CCL4, PIK3CD known to be related with tumor malignancy were significantly up regulated in TCL compared to BCL (Table 1).

Conclusions and clinical importance

Significant DEGs were identified between TCL and BCL, and these DEGs are presumed to have been related to poor treatment response and prognosis of TCL. This is the first study of transcriptome profiling between normal and lymphoma in the comparison of TCL and BCL in dogs.