Veterinarian College of Veterinary Medicine, Chungbuk National University Chungju, Ch'ungch'ong-bukto, Republic of Korea
Abstract:
Background: Neuroinflammation is known as the cause and result of seizure and epilepsy. Hypothesis/
Objectives: This study aimed to identify hematologic inflammatory parameter (HIP) and inflammatory mediators that change after single seizure in the canine pentylenetetrazole (PTZ)-induced seizure model. Animals: Five healthy Beagle dogs were included in this study.
Methods: The 3% PTZ solution was infused until generalized convulsion occurred. Blood sampling time points were divided into two periods; short period (baseline, 30, 60, 90, and 120 min after seizure induction) and long period (baseline, 2, 6, 12, 24, and 48 h after seizure induction). HIP was calculated and serum prostaglandin E2 (PGE2) and leukotriene B4 (LTB4) concentrations were estimated using the enzyme-linked immunosorbent assay.
Results: Significant changes (P < 0.05) in each HIP were observed at each time point; NM (90 min), NLR (60, 90, and 120 min), LMR (60, 90, 120 min, 2, 12, and 24 h), PAR (90 min), LA (60, 90, 120 min, and 2 h), and NP (6 h). After seizure induction, LTB4 significantly increased (P < 0.05) at 60, 90 min, 2, 6, and 48 h. PGE2 significantly increased only 6 h after seizure induction (P < 0.05). The LA was identified as an LTB4-correlated biomarker and showed significant changes in long-period experiment (P < 0.05, r = -0.4194). Conclusion and clinical importance: The LA was the only HIP that the 5-lipoxygenase pathway may be related with seizure associated neuroinflammation.
