Abstract: Background: Syndecan-1 (SDC1) is an established marker of endothelial glycocalyx (EG) shedding. Most of the research on SDC1 has focused on plasma or serum, and little is known about urinary levels.
Objectives: To measure urinary SDC1 in dogs undergoing anesthesia with either sevoflurane or isoflurane; to compare urinary SDC1 to length of anesthesia and quantity of IV crystalloids.
Animals: Thirty-one client-owned dogs undergoing anesthesia for MRI +/- surgery for suspected intervertebral disc disease (IVDD).
Methods: Dogs with suspected IVDD were randomized to undergo anesthesia with either sevoflurane or isoflurane. Urine was collected prior to, and immediately after anesthesia for analysis of SDC1. Urinary creatinine levels were also measured and the ratio of urinary SDC1 to urinary creatinine (USCR) was used to account for dilution.
Results: Median (range) urinary SDC1 was significantly higher after anesthesia compared with baseline for all groups combined (p< 0.05). There was no significant difference between groups for age, sex, weight, and type of anesthesia. A multiple regression analysis of the effect of the independent variables inhalant type, age, weight, sex, anesthesia time, surgery, and quantity of IV fluids on the dependent variable, SDC1, found that only quantity of IV fluids significantly predicted a change (p< 0.0001).
Conclusions and clinical importance: Total volume of crystalloid fluids administered to anesthetized dogs may impact SDC1 in urine. Further investigations are warranted to evaluate the relationship between IV fluids and vascular/urinary SDC1.
